Task 3 Single cell PCR and DNA sequencing of identified cells

Background: John, Lundholm and Edvardsen have ample experience with single cell PCR of protists.

Aim: Obtain more rDNA reference sequences from protists that have not yet been cultivated or sequenced.

Planned work: Pipette live, single protist cells from net and concentrated water samples, especially small and heterotrophic. Characterise morphology of the cells by microscopy and micrographs. Determine rDNA sequences of marker genes (SSU and LSU rDNA) from single cells by PCR and DNA Sanger sequencing.